Table 2. Primers used to clone promoter constructs.

The KpnI sites in the forward primers and the NheI sites in the reverse primers are underlined. The position of the primers is in relation to the AUG initiation codon in the ZAN/U strain.

Primer name	Sequence (5′→3′)	Position	Construct name
PG3F1	GGACAGGTACCGGTTGGATGGGATTTTTCTTCGCG	−1303/−1269	PGZa3-1; PGKi3-1
PG3F2	CAAGGGTACCATTTCACAGCGATCGTG	−1215/−1189	PGZa3-2; PGKi3-2
PG3F3	CGGACGGTACCCATATTTACCTCAATTCACC	−1050/−1021	PGZa3-3; PGKi3-3
PG3F4	GCTCAGGTACCTAAACAATCGATAATTAAACTGC	−1007/−974	PGZa3-4; PGKi3-4
PG3F5	GCTCGGTACCTGCGTAAGACTTTGTAGC	−671/−644	PGZa3-5; PGKi3-5
PG3F6	CAATGGGTACCGAGCGATCGACTCTGAACAC	−339/−309	PGZa3-6; PGKi3-6
PG3R	GTGCCATGCTAGCTCGTTGTTGTTGTTGTTGATG	−27/+7
PG12F1	GTAACAGGTACCTGCTATTCGCGGTTGTTAAC	−356/−325	PGZa12-1; PGKi12-1
PG12F2	GTGTAGGTACCAAAGCACCTAGTAACG	−312/−286	PGZa12-2; PGKi12-2
PGZa12F6	GTGTAGGTACCAAAGCACCTAGTAACGTTTTTCTTGTGCATAAAAAACAGGAATTCG	−312/−256	PGKiMu
PGki12F6	GTGTAGGTACCAAAGCACCTAGTAACGTTTTTCTTGTGCATAAAAAAAACAGGAATTCG	−312/−256	PGZaMu
PG12F3	CAGGGGTACCCTTCTGCTTTTATGTTGCAGTACG	−265/−232	PGZa12-3; PGKi12-3
PG12F4	GAACCGGTACCAGTATGAAATAAATTCGCCG	−229/−199	PGZa12-4; PGZa12-4
PG12R2	CAAGAGCTAGCCCCCGAACTGATGATTGGCTGTG	−79/−46	PGZa12-5,12-6; PGKi12-5,12-6
PG12R	GGACATGCTAGCAGCGAACTAAAAACTGGATGG	−27/+6
PG71F	CTGCTGGGTACCATCGCTCAGCCTGAAACGCG	−766/−735
PG71R	GGCATGCTAGCGACTGGGGGTTTCGTCTACG	−26/+5
PG17F	CTGCTGGCTAGCATCGCTCAGCCTGAAACGCG	−766/−735
PG17R	GGCATGGTACCGACTGGGGGTTTCGTCTACG	−26/+5
PG45F	GTGAACGGTACCTGGTTGAGTGAACGCTTCAAGC	−304/−271
PG45R	CGTTGCGCTAGCTCCGTCGTGATGAGCGGTTCAAG	−26/+9