Table 2. High-molecular-mass glycoproteins in SMG secretions.
Proteins were separated by 1D SDS/Ag/PAGE, digested with trypsin, and identified by MALDI-TOF-MS and PMF. MUC5B and MUC5AC were detected in a protein band at ≈4 MDa, and gp-340 variant protein was detected in a protein band at ≈1 MDa. The peptide coverage (%) for each protein is shown for each subject.
| Peptide coverage (%) | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CF lung disease | Non-CF lung disease | Control | |||||||||||||
| Protein band | Identified protein | SwissProt accession no. | 1 | 2 | 3 | 4 | 5 | 1 | 2 | 3 | 4 | 5 | 1 | 2 | 3 |
| ≈4 MDa | Mucin 5B | Q9HC84 | 10.6 | 7.1 | 7.1 | 9.2 | 7.2 | 10.3 | 7.7 | 13.2 | 9.2 | 8.7 | 9.9 | 10.3 | 14.7 |
| Mucin 5AC | Q8WWQ4 | 14.7 | N.D.* | 8.0 | 12.6 | 11.0 | 14.0 | 4.8 | 6.4 | 9.3 | 8.0 | 6.4 | 12.2 | 9.2 | |
| ≈1 MDa | gp-340 | Q9UKJ4 | 9.7 | 6.9 | N.D. | N.D. | 12.0 | 11.4 | 20.7 | 19.9 | 22.8 | 23.5 | 29.2 | 17.2 | 28.8 |
* N.D., not detected.