Representative proteins and their predominant glycosylation patterns associated with specific subcellular compartments, along with the pros and cons. (A) Representative predominant glycosylation patterns in the specific subcellular compartments. GlcNAc (Gn), N-acetylglucosamine; Man (M), mannose; Xyl (X), xylose; Fuc (F), fucose; Gal, galactose, Lea, Lewis a. (B) Representative proteins in specific subcellular compartments. The Cryo-EM data of representative VLPs and the PDB structure of representative proteins are flanked with the 4 subcellular compartments. (C) The pros and cons of different subcellular accumulation in tobacco. PTM, posttranslation modification; CT, chloroplast transformation; NT, nuclear transformation.