Table 3.

List of recombinant proteins accumulated in tobacco chloroplast

Protein	Expression pattern	Transit peptide	Expression level	Tobacco species	References
β-Glucosidase	Chloroplast		75% TSP	N. tabacum	[66]
TetC-Cel6A	Chloroplast		28% in green leaves	N. tabacum	[67]
SOD	Chloroplast		9% TSP	N. tabacum	[70]
EGF	Chloroplast		1.57±0.05 g/kg FW	N. tabacum	[72]
CTB	Chloroplast		7.49 mg/g FW	N. tabacum	[73]
HPV L1 VLP	Chloroplast		24% TSP	N. tabacum	[74]
HIV-1 p24	Nucleus	rbcS1-TP	1 mg/kg FW	N. benthamiana	[76]
HPV-16 L1 VLP	Nucleus	rbcS1-TP	6–8 mg/kg FW	N. benthamiana	[79]
HPV-16 L1 VLP	Nucleus	rbcS1-TP	11% TSP	N. benthamiana	[77]
HPV-16 L1/L2 chimera	Nucleus	rbcS1-TP	1.2 g/kg FW	N. tabacum	[78]

TetC-Cel6A, endoglucanase Cel6A; EGF, epidermal growth factor; CTB, cholera toxin B subunit; SOD, superoxide dismutase; HPV L1 VLP, human papillomavirus L1 virus like particle; HIV-1 p24, human immunodeficiency virus type I p24 capsid subunit; HPV-16 L1/L2 chimera, human papillomavirus type 16 L1/L2 chimeras; rbcS1-TP, transit peptide from rbcS1