. 2024 Aug 6;14(16):2290. doi: 10.3390/ani14162290

Table 3.

Percentage of total motility, viability, lipid peroxidation (MDA), and pH of semen diluted with extender supplementing different levels of AV gel and stored for 72 h at 5 °C.

Time of Storage (h)	Treatments								SEM
Time of Storage (h)	Control	0.25%	0.50%	1.0%	2.5%	5.0%	10%	20%	SEM
Sperm motility
T0	85.67 ^cw	89.37 ^bw	94.83 ^aw	95.88 ^aw	82.21 ^dw	78.54 ^ew	72.17 ^fw	65.88 ^gw	0.70
T24	80.54 ^dx	88.75 ^ax	83.21 ^cx	85.54 ^bx	74.97 ^ex	74.43 ^ex	69.75 ^fx	55.63 ^gw
T48	71.60 ^cy	74.24 ^by	75.62 ^by	79.00 ^ay	72.54 ^cy	70.67 ^dy	62.29 ^ey	42.62 ^fy
T72	65.62 ^dz	71.79 ^cz	74.42 ^bz	77.88 ^az	61.92 ^ez	57.87 ^fz	51.13 ^gz	32.54 ^hz
Sperm viability
T0	92.46 ^cw	95.67 ^bw	96.2 ^bw	98.83 ^aw	91.33 ^cw	82.96 ^dw	74.04 ^ew	72.33 ^fw	0.53
T24	85.21 ^cx	90.67 ^bx	92.42 ^bx	95.50 ^ax	81.42 ^dx	77.83 ^ex	77.63 ^ex	62.54 ^fx
T48	74.68 ^cy	77.29 ^by	79.17 ^ay	80.67 ^ay	74.08 ^cy	72.63 ^dy	63.92 ^ey	45.75 ^fy
T72	71.88 ^cz	75.33 ^bz	75.97 ^bz	79.17 ^az	74.88 ^bz	62.46 ^dz	55.04 ^ez	34.13 ^fz
MDA
T0	1.09 ^dz	0.68 ^ez	0.59 ^gz	0.54 ^hz	0.62 ^fz	1.15 ^cz	1.24 ^bz	1.35 ^az	0.02
T24	2.14 ^dy	1.09 ^fy	1.09 ^fy	1.09 ^fy	2.07 ^ey	3.33 ^cy	3.56 ^by	3.79 ^ay
T48	4.23 ^cx	3.09 ^ex	3.06 ^ex	3.04 ^ex	4.06 ^dx	5.57 ^bx	5.90 ^ax	5.92 ^ax
T72	5.73 ^dw	5.49 ^ew	5.35 ^fw	5.26 ^gw	5.34 ^fw	5.94 ^cw	7.33 ^bw	7.66 ^aw
pH
T0	7.00 ^aw	7.00 ^aw	7.00 ^aw	7.00 ^aw	6.99 ^abw	6.99 ^abw	6.98 ^abw	6.98 ^abw	0.04
T24	6.99 ^abx	6.99 ^abx	6.99 ^abx	6.99 ^abx	6.95 ^cx	6.89 ^dx	6.84 ^ex	6.82 ^efx
T48	6.94 ^ay	6.94 ^ay	6.93 ^aby	6.93 ^aby	6.88 ^ey	6.81 ^fy	6.74 ^hy	6.73 ^hy
T72	6.91 ^az	6.90 ^abz	6.90 ^abz	6.90 ^abz	6.84 ^dx	6.72 ^fz	6.63 ^hz	6.61 ^hz

SEM, standard error of the means. ^{a, b, c, d, e, f, g, h} (different letters within a row) and ^{w, x, y, z} (different letters within a column) indicate the interaction between treatment effects and time to storage effect significant differences (p < 0.05).