Figure 2.

The purification of the AMP derived from NNS4-3 separated by RPC with gradient elution of mobile phase B (green solid line) showed that the major peak (arrow) was the active fraction (a). SDS-PAGE with 15% gel was performed for the characterization of protein bands stained with coomassie brilliant blue (b). The active fraction from each purification step, as followed by RPC (Lane 1), protein precipitation (Lane 2), and CFS collected from NNS4-3 culture (Lane 3), was electrophoresed and compared with the protein marker (Lane M). The protein bands exhibiting antibacterial activity were observed as an inhibition zone when overlaid with soft agar containing MRSA strain 2468 (c). The correlation between protein bands in the stained gel and the inhibition zone in the soft-agar overlaid gel in Lane 1 (solid arrow) and Lane 2 (dashed arrow) was found at a similar position.