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. 2024 Jul 30;13(8):716. doi: 10.3390/antibiotics13080716

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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The ortholog comparison of BGCs between Bacillus paralicheniformis NNS4-3 and the reference Bacillus licheniformis ATCC 10716. The prediction showed that the coding gene of NNS4-3 displayed 100% similarity to gene orthologs that were a cluster of bacitracin-like biosynthetic genes against the reference cluster. The function of predicted domain was represented by the color code in the AntiSMASH cluster visualization system (a). The encoded proteins from each gene that were responsible for AMP synthesis by modular enzymes (b). The differences in amino acid residues of each module enzyme (below) were compared to the reference (above), which is indicated by the red color along the amino acid sequences. The amino acid sequence similarity of each coding protein is presented with identity (%), coverage (%), and number of mismatches.