TABLE 2.
Transformation efficiency of strain 26695 with PCR products of the pbp1 gene from AMXR strain KIN76
| Fragment | Mutated allele(s) | AMX concentration on plate (µg/mL) | Transformation efficiencya |
|---|---|---|---|
| No DNA control | None | 0.125 | >104 |
| 0.25 | 0 | ||
| Fr0 | None | 0.125 | >104 |
| 0.25 | 0 | ||
| Fr1 | T558S | 0.125 | >104 |
| 0.25 | 0 | ||
| Fr2 | N562H | 0.125 | >104 |
| 0.25 | 0 | ||
| Fr6 | T593A | 0.125 | >104 |
| 0.25 | 0 | ||
| Fr7 | G595S | 0.125 | >104 |
| 0.25 | 0 | ||
| Fr3 | T558S/N562H | 0.25 | 2.7 × 103 |
| 0.5 | 0 | ||
| Fr4 | T593A/G595S | 0.25 | 3.5 × 103 |
| 0.5 | 0 | ||
| Fr5 | T558S/N562H/T593A/G595S | 0.125 | >104 |
| 0.25 | 5.4 × 103 | ||
| 0.5 | 3.6 × 103 |
a
Transformation efficiency (transformants/microgram DNA) calculated based on the formula shown in Materials and Methods.