Figure 5.

ROT or ROA on neuronal markers. Cells incubated with ROT or ROA for 48 h were collected, cell lysates were prepared, and the levels of NeuN/GAPDH ((A) F_(8,18) = 8.669, p < 0.0001, R² = 0.7939), NF-H/GAPDH ((B) F_(8,18) = 4.961, p = 0.0023, R² = 0.6880), and β3-Tubulin/GAPDH ((C) F_(8,18) = 36.19, p < 0.0001, R² = 0.9415) protein expression were assessed by Western blotting. Original uncut Western blot images are shown in Supplementary Figure S10. The data are expressed as mean ± SEM with n = 3 and analyzed by one-way ANOVA followed by Tukey’s post hoc test. * p < 0.05, ** p < 0.01, and *** p < 0.001 significantly different from control cells.