Table 4.
Iridoids profiling in original bitter tincture (BT), conventional hydroethanolic extraction (CE), hydroethanolic ultrasound-assisted extraction (EtOH UAE), choline chloride/citric acid ultrasound-assisted extraction (ChCl:CA UAE), choline chloride/lactic acid ultrasound-assisted extraction (ChCl:LA UAE), and sucrose/citric acid ultrasound assisted extraction (Su:CA UAE).
| Iridoids | Linear Equation | R2 | tR | LOD | LOQ | Concentration | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| BT 1 | CE 1 | EtOH UAE 1 |
ChCl:CA 1 UAE |
ChCl:LA 1 UAE |
Su:CA 1 UAE |
||||||
| Swertiamarin | y = 7 × 106x + 1071.2 | 0.999 | 10.653 | 0.008 | 0.025 | 1.886 ± 0.013 | 0.0366 ± 0.002 | 0.406 ± 0.018 | 0.112 ± 0.014 | 1.626 ± 0.020 | n.d. |
| Gentiopicroside | y = 3 × 106x + 20,893 | 0.999 | 11.523 | 0.012 | 0.037 | 1.076 ± 0.016 | 0.237 ± 0.000 | 0.288 ± 0.020 | 0.076 ± 0.005 | 2.506 ± 0.001 | n.d. |
| Sweroside | y = 3 × 106x + 5645.2 | 0.999 | 12.006 | 0.009 | 0.029 | 1.211 ± 0.005 | 3.119 ± 0.056 | 0.968 ± 0.108 | 0.274 ± 0.028 | 1.664 ± 0.074 | n.d. |
| Amarogentin | y = 1 × 107x − 16,003 | 0.999 | 19.6967 | 0.010 | 0.032 | 0.433 ± 0.005 | 0.651 ± 0.005 | 0.340 ± 0.083 |
0.182 ± 0.064 | 0.304 ± 0.001 | n.d. |
1 result expressed in mg/L as the average of at least a triple injection of the sample ± SD.