Fig. 2.

Activation of the unfolded protein response but no unresolved ER stress in the retina and optic nerve of Wfs1 KO mice. a, b Elevated BiP levels indicate that there is accumulation of unfolded proteins in the ER lumen in the retina (a) and optic nerve (b) of Wfs1 KO mice, but not of WT controls; starting from the age of 4.5 and 3 months, respectively. Two-way ANOVA with Sidak’s multiple comparisons; F_1,40=55.52 and p < 0.0001 for genotype, F_3,40=2.992 and p = 0.0421 for age, with p = 0.0997 for 3 months, p = 0.0061 for 4.5 months, p < 0.0001 for 6 months, p < 0.0001 for 7.5 months, for retina; F_1,39=67.94 and p < 0.0001 for genotype, F_3,39=6.239 and p = 0.0015 for age, with p = 0.0022 for 3 months, p = 0.0015 for 4.5 months, p = 0.0956 for 6 months, p < 0.0001 for 7.5 months, for optic nerve. c, d CHOP expression levels are similar for both genotypes in the retina (c) and optic nerve (d). Two-way ANOVA with Sidak’s multiple comparisons; F_1,39=2.763 for genotype, F_1,19=8.846 and p = 0.0036 for age, for retina; F_1,10=0.5544 for genotype, F_3,30=0.3121 for age, for optic nerve. Data presented as mean ± SEM, N = 6. TP: total protein stain