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. 2024 Aug 6;12(8):1771. doi: 10.3390/biomedicines12081771

Figure 2.

Figure 2

Effect of DDW on the membrane translocation of GLUT4. Fully differentiated C2C12 myotubes were cultured for 48 h in media containing various concentrations of DDW. After insulin stimulation to induce the membrane translocation of GLUT4, the cells were harvested and membrane proteins were extracted. GLUT4 protein was detected and quantified using Western blotting. (a) Western blotting of the membrane protein fraction; (b) GLUT4 bands were quantified using ImageJ. Values relative to those in cultures without insulin in natural water medium (150 ppm) are shown. The sample size was four. * Significant difference between the presence and absence of insulin in DDW medium at the same concentration (p < 0.05).