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Schematic of steps involved in PacBio sequencing of Ig genes from myeloblasts and B cells. Bone marrow mononuclear cells were obtained from AML patients. Myeloblasts and B cells were sorted by fluorescence-activated cell sorting. Total mRNA was extracted and reverse transcribed by 5′-RACE, and the Ig heavy chain and light chain were amplified by multiplex PCR. Through library construction, PacBio sequencing, and data processing, the Ig repertoire was obtained and analyzed.
