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. 2024 Jul 23;13(8):887. doi: 10.3390/antiox13080887

Figure 1.

Figure 1

In vitro differentiation of BMDCs into macrophages. (A) Representative flow cytometry pseudocolor plots for BMDCs following culture with M-CSF and the addition of LPS at 96 h. CD45+ gated cells were further analyzed for the expression of CD11b + F4/80+ cells, the percentage of which is shown in each histogram. Unpaired t-test was performed between the two groups. (B) Graphical representation of the percentages of macrophages in vehicle and THC-treated groups at 120 h by flow cytometry. (C) Representative flow cytometry pseudocolor plot for CD45 + CD11b + F4/80+ macrophages cultured with different concentrations of THC. (D) Graphical representation of the percentages of macrophages in THC dose-dependent response assay. (E) Cell viability as measured by cell counter at 48, 72, and 120 h. Two-way ANOVA was performed. (A–E) n = 3, levels of statistical significance were assigned according to the following cutoffs: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.