Table 2.
Box-Behnken experimental design (BBD) presenting the outcome of independent variables on dependent variables.
| Independent Variable | Dependent Variable (Response) | |||||
|---|---|---|---|---|---|---|
| Formulation Code |
Factor 1 (A) | Factor 2 (B) | Factor 3 (C) | PS (nm) Y1 |
EE (%) Y2 |
pH Sensitivity (%) Y3 |
| pHLip1 | 1 | 0 | 1 | 202.5 ± 1.3 | 89.3 ± 2.0 | 76.3 ± 0.5 |
| pHLip 2 | −1 | 0 | 1 | 170.3 ± 0.8 | 66.0 ± 2.2 | 60.7 ± 1.1 |
| pHLip 3 | 0 | 1 | −1 | 143.2 ± 1.2 | 78.4 ± 1.6 | 76.0 ± 2.4 |
| pHLip 4 | 0 | 0 | 0 | 165.5 ± 1.8 | 90.4 ± 2.0 | 78.2 ± 2.6 |
| pHLip 5 | −1 | 1 | 0 | 160.3 ± 1.9 | 77.5 ± 0.8 | 70.2 ± 1.90 |
| pHLip 6 | −1 | 0 | −1 | 204.1 ± 0.9 | 85.6 ± 1.1 | 56.4 ± 1.3 |
| pHLip 7 | 0 | 1 | 1 | 166.9 ± 1.2 | 69.2 ± 1.2 | 73.8 ± 2.0 |
| pHLip 8 | 1 | 1 | 0 | 126.7 ± 1.5 | 88.7 ± 1.6 | 90.1 ± 0.8 |
| pHLip 9 | −1 | −1 | 0 | 231.8 ± 2.1 | 81.3 ± 0.6 | 61.9 ± 1.3 |
| pHLip 10 | 0 | −1 | 1 | 326.4 ± 1.2 | 75.7 ± 1.5 | 68.7 ± 1.7 |
| pHLip 11 | 1 | −1 | 0 | 320.2 ± 1.3 | 93.7 ± 1.1 | 74.3 ± 1.5 |
| pHLip 12 | 0 | 0 | 0 | 177.7 ± 0.3 | 89.2 ± 1.8 | 87.6 ± 0.4 |
| pHLip 13 | 0 | −1 | −1 | 316.9 ± 1.3 | 82.3 ± 0.5 | 64.8 ± 1.4 |
| pHLip 14 | 0 | 0 | 0 | 188.5 ± 1.7 | 90.6 ± 1.4 | 78.0 ± 1.7 |
| pHLip 15 | 1 | 0 | −1 | 190.8 ± 2.4 | 90.8 ± 1.6 | 77.4 ± 1.2 |