Figure 3.

Induction of SASP and growth arrest in axitibin-treated A549 cells. (A) Three lung cancer cell lines were cultured with either axitinib or lenvatinib (2.5 μM) for 3 days. Expression levels of p21 and IL-8 mRNAs were determined in the harvested cells. Data are means ± SD of three samples. Means were compared using Student’s t-test (** p < 0.01 compared to the untreated control). (B) A549 cells were cultured with axitinib or lenvatinib (2.5 μM) for 3 days. Then, these cells were stained with APC-conjugated anti-Ki-67 antibody or APC-conjugated anti-BrdU antibody (green line) and analyzed by flow cytometry. The red line is an isotype-matched control antibody.