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. 2024 Aug 14;46(8):8835–8851. doi: 10.3390/cimb46080522

Figure 1.

Figure 1

Three expression constructs containing GFP-SIRT1 isoforms. The v1 coding region sequence is 747 amino acids (AAs) (A), the v2 coding region sequence is 452 AAs (B), and the v3 coding region is 444 AAs (C). The SIRT1 protein expression was present in wild-type cells, but absent in SIRT1-KO cells (D). The SIRT1 isoform proteins were detected in the SIRT1-KO cells after transfection of empty vector (EV) and SIRT1 isoform plasmid vectors (E). The relative protein levels of SIRT1 isoform proteins are shown in (F). Data are mean ± SD of three repeats. Significant difference *** p < 0.001 by one-way ANOVA with Tukey’s procedure.