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. 2024 Aug 14;46(8):8835–8851. doi: 10.3390/cimb46080522

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Immunoprecipitation and western blot analysis. (A) Twenty-four hours post-transfection with SIRT1 isoforms, C2C12 cell lysate analyzed for histone-H4 acetylation. Immunoblot represents histone-H4-acetylated lysine at K5, K8, K12, and K16, respectively. Total histone H4 protein and β-actin were used as loading controls. (B) SIRT1-v1 increases the NT-PGC1α protein expression; representative western blot of NT-PGC1α with different SIRT1-v1 isoforms [27,33]. GAPDH was used as a loading control. Blots are representative of three repeats.