Fig. 8. MS41 potently depletes ENL protein with YEATS domain mutations.

(A) Schematic of ENL YEATS domain mutants T1 (small insertion) and T3 (small deletion) identified in Wilms’ tumor. (B) Immunoblots for ENL and β-actin in HEK293 cells stably expressing endogenous level of wild-type (WT) or YEATS mutant ENL (T1 and T3) treated with the indicated concentrations of MS41 for 24 hours. (C and D) RT-qPCR analysis showing mRNA expression levels of ENL target genes HOXA11 (C) and HOXA13 (D) in HEK293 stable cells as in (B) treated with DMSO, MS41 (100 nM), MS41N (100 nM), or PFI-6 (100 nM) for 72 hours. Error bars represent means ± SEM from three independent experiments (Student’s t test). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.