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. 1988 Nov 15;256(1):69–73. doi: 10.1042/bj2560069

A spectrophotometric assay for 6-phosphogluconolactonase involving the use of immobilized enzymes to prepare the labile 6-phosphoglucono-delta-lactone substrate.

R D Moir 1, G B Stokes 1
PMCID: PMC1135369  PMID: 3223913

Abstract

We report the development of a new spectrophotometric assay for 6-phosphogluconolactonase. The labile substrate 6-phosphoglucono-delta-lactone is prepared from glucose 6-phosphate by enzymes co-immobilized on Sepharose beads. The assay has the advantages of high sensitivity for routine determination of enzyme activity and allows determination of both Km and Vmax. from a single assay. A method for estimating the contribution of spontaneous hydrolysis to total hydrolysis is described. This assay overcomes the problems encountered with all previous assays.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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