Representative double immunofluorescence staining of the substantia nigra of an aminoindan-treated double- lesioned rat. (A) Hsp60 and TH: Dopaminergic neurons in the pars compacta observed with TH staining co-expressed Hsp60. TH labeling shown in green on the middle panel, Hsp60 shown in red in the left panel, and overlay shown on the right panel. (B) Hsp60 and NeuN: In the SN pars reticulata, interneurons were visualized with NeuN (green, center panel) and co-labeling for Hsp60 (red, left panel) was observed. Overlay is shown on the right panel. Scale bar in (C) represents 100 μm.