Abstract
The phosphate complex of sulphite oxidase in the Mo(V) oxidation state was investigated by e.p.r. spectroscopy. Third-derivative spectra reveal a wealth of structural detail previously unobserved in this spectrum. Most notable is the presence of hyperfine coupling from two inequivalent I = 1/2 nuclei, which we tentatively attribute to two 31P nuclei. Unresolved hyperfine interactions from at least one exchangeable 1H nucleus are also present.
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Selected References
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- Bray R. C., Gutteridge S., Lamy M. T., Wilkinson T. Equilibria amongst different molybdenum (V)-containing species from sulphite oxidase. Evidence for a halide ligand of molybdenum in the low-pH species. Biochem J. 1983 Apr 1;211(1):227–236. doi: 10.1042/bj2110227. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bray R. C. The inorganic biochemistry of molybdoenzymes. Q Rev Biophys. 1988 Aug;21(3):299–329. doi: 10.1017/s0033583500004479. [DOI] [PubMed] [Google Scholar]
- Bray R. C. The reactions and the structures of molybdenum centers in enzymes. Adv Enzymol Relat Areas Mol Biol. 1980;51:107–165. doi: 10.1002/9780470122969.ch3. [DOI] [PubMed] [Google Scholar]
- George G. N., Bray R. C. Studies by electron paramagnetic resonance spectroscopy of xanthine oxidase enriched with molybdenum-95 and with molybdenum-97. Biochemistry. 1988 May 17;27(10):3603–3609. doi: 10.1021/bi00410a011. [DOI] [PubMed] [Google Scholar]
- Gutteridge S., Lamy M. T., Bray R. C. The nature of the phosphate inhibitor complex of sulphite oxidase from electron-paramagnetic-resonance studies using oxygen-17. Biochem J. 1980 Oct 1;191(1):285–288. doi: 10.1042/bj1910285. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johnson J. L., Hainline B. E., Rajagopalan K. V., Arison B. H. The pterin component of the molybdenum cofactor. Structural characterization of two fluorescent derivatives. J Biol Chem. 1984 May 10;259(9):5414–5422. [PubMed] [Google Scholar]
- Kessler D. L., Rajagopalan K. V. Hepatic sulfite oxidase. Effect of anions on interaction with cytochrome c. Biochim Biophys Acta. 1974 Dec 29;370(2):389–398. doi: 10.1016/0005-2744(74)90100-4. [DOI] [PubMed] [Google Scholar]
- Kessler D. L., Rajagopalan K. V. Purification and properties of sulfite oxidase from chicken liver. Presence of molybdenum in sulfite oxidase from diverse sources. J Biol Chem. 1972 Oct 25;247(20):6566–6573. [PubMed] [Google Scholar]
- Lamy M. T., Gutteridge S., Bary R. C. Electron-paramagnetic-resonance parameters of molybdenum(V) in sulphite oxidase from chicken liver. Biochem J. 1980 Feb 1;185(2):397–403. doi: 10.1042/bj1850397. [DOI] [PMC free article] [PubMed] [Google Scholar]