Skip to main content
. 2024 Aug 8;25(16):8655. doi: 10.3390/ijms25168655

Figure 1.

Figure 1

The UPLC chromatograms of SBL. The UPLC analysis was conducted using an Agilent 1290 UHPLC system coupled with a 6350 QTOF system (Santa Clara, CA, USA) and an Agilent ZORBAX Eclipse Plus RRHD column (2.1 mm × 150 mm, 1.8 μm). The chromatographic separation was maintained at 40 °C with gradient conditions at a 0.4 mL/min flow rate. Elution was performed with a mobile phase of A (0.1% formic acid in deionized and distilled water) and B (0.1% formic acid in acetonitrile) under a gradient program of 95% A at 0–2 min, 70–95% A at 2–9 min, 48–70% A at 9–10 min, 32–48% A at 10–12 min, 20–32% A at 12–13 min, and 20% A at 13–17 min, followed by 0–20% A at 17–19 min. Data were collected between 1 and 19 min.