Table 2.

Primer lists and optimized conditions.

Name	Sequence 5′ --> 3′	Amplicon Size (bp)	Optimized Condition
COL3A1_Ex1F	GCAGGGAACAACTTGATGGTGC	1079	58 °C 30 min
			94 °C 2 min
			94 °C 10 s
COL3A1_Ex14R	GGTTGACCATCACTGCCTCGA		58 °C 10 s
			68 °C 1 min
			for 20 cycles
COL3A1_Ex9F	GACCTGGAGAGCGAGGATTG	687	58 °C 30 min
			94 °C 2 min
			94 °C 10 s
COL3A1_Ex20R	CCTTGCCATCTTCGCCTTTA		58 °C 10 s
			68 °C 1 min
			for 20 cycles
hGAPDH Ex1F	ATGCTGGCGCTGAGTACGT	874	94 °C 2 min
			94 °C 30 s
hGAPDH Ex3R	AGGGGTCTACATGGCAACTG		58 °C 30 s
			68 °C 1 min
			for 20 cycles