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. 2024 Aug 16;25(16):8926. doi: 10.3390/ijms25168926

Figure 3.

Figure 3

Human Wharton jelly-derived mesenchymal stromal cells (cMSC(WJ)s) identification. (A–F) differentiation. Oil Red O-stained hMSC(WJ)s cultured in (A) adipogenic medium. Lipid droplets stained in red. and (B) in standard growth medium (CTRL); Alizarin Red stained hMSC(WJ)s cultured in (C) osteogenic medium. Calcium deposits stained in red and (D) in GM; (E) chondrogenic differentiation, toluidine blue staining. Proteoglycans stained in purple; (F) immunocytochemistry. hMSC(WJ) stained for the presence of vimentin intermediate filament expressed in mesenchymal cells (G) Flow cytometry analysis of surface antigens: cells are positive for CD90, CD44, CD73, CD105. Negative cocktail (MIX neg) consisted of CD34, CD45, CD11b, CD19 and HLA-DR. Individual plots from representative population, Scale bars: 20 µm (A,B); 50 µm (C–E); 1000 μm (F).