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. 2024 Aug 16;25(16):8926. doi: 10.3390/ijms25168926

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Canine Wharton jelly-derived mesenchymal stromal cells (cMSC(WJ)s) identification. (A–E) differentiation. Oil Red O-stained cMSC(WJ)s cultured (A) in adipogenic medium, lipid droplets are red and (B) in standard growth medium (CTRL); Alizarin Red stained cMSC(WJ)s cultured (C) in osteogenic medium, calcium deposits are red and (D) in GM; (E) chondrogenic differentiation, toluidine blue staining, proteoglycans stained in purple; (F) immunocytochemistry. cMSC(WJ) stained for the presence of vimentin intermediate filament expressed in mesenchymal cells; (G) Flow cytometry analysis of surface antigens: cells are positive for CD90, CD44, negative for CD11b, CD45; Scale bars: 20 µm (A,B); 50 µm (C–E); 1000 µm (F).