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. 2024 Aug 8;25(16):8677. doi: 10.3390/ijms25168677

Figure 5.

Figure 5

Mitochondrial oxygen consumption was measured with a Clark-type electrode. The energization was obtained with (A) glutamate/malate (G/M) and 175 nmol ADP or (B) succinate (SUC), rotenone and 125 nmol ADP. State 3 represents the maximum consumption of oxygen in the presence of ADP, and state 4 represents oxygen consumption without ADP consumption (both expressed as nanoatoms of oxygen/min*mg protein). The respiratory control ratio (RCR) determines the coupling between substrate oxidation and oxidative phosphorylation. ADP/O is the efficiency of oxidative phosphorylation and is expressed as nmol ADP/nanoatom of oxygen. The data are expressed as the means ± SDs that were calculated from at least three separate measurements. WC—untreated wild-type; WM—wild-type plus melatonin; ObC—untreated ob/ob; ObM—ob/ob plus melatonin. Statistical comparisons: # wild-type vs. ob/ob and * treated with melatonin vs. untreated counterpart. The number of symbols indicates the level of statistical significance: # for p < 0.050, ## for p < 0.010, and *** for p < 0.001.