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. 2024 Aug 8;25(16):8677. doi: 10.3390/ijms25168677

Figure 6.

Figure 6

Mitochondrial and cellular energy status. (A) Bar chart showing the semiquantitative optical density (arbitrary units of blot bands) and representative Western blot image of adenosine monophosphate-activated protein kinase (AMPK) and its phosphorylated form (p-AMPK). (B) Basal mitochondrial and cytosolic ATP content and ATP produced after energization with glutamate/malate (G/M) and succinate (SUC), which was calculated as the difference between the ATP content in mitochondria after respiration and basal mitochondrial ATP. The ATP content is presented as pM ATP/mg protein. The data are expressed as the means ± SDs that were calculated from at least three separate measurements. WC—untreated wild-type; WM—wild-type plus melatonin; ObC—untreated ob/ob; ObM—ob/ob plus melatonin. Statistical comparisons: # wild-type vs. ob/ob and * treated with melatonin vs. untreated counterpart. The number of symbols indicates the level of statistical significance: * for p < 0.050, **/## for p < 0.010, and ***/### for p < 0.001.