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Isolated protein band from silver-stained gel. HMC3 cells (1 × 106 cells) were stimulated with or without 1 μg/mL of 27OHChol (concentration of stock: 2 mg/mL) for 48 h, and then surface proteins were isolated with PIERCE® Cell Surface Protein Isolation Kit. The proteins were separated in 10% SDS-contained polyacrylamide gel, and then the gel was stained with PIERCE® Silver Stain Kit. A band was isolated from the gel (red square) and analyzed by MALDI-TOF-MS/MS.
