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. 2024 Aug 6;25(16):8590. doi: 10.3390/ijms25168590

Figure 7.

Figure 7

CHD1Li-mediated PAR translocation to the cytoplasm activates PARthanatos. (A) Intensity of cytoplasmic PAR in SUM149PT cells treated for 6 h with OTI-611, olaparib, AZD5305, or doxorubicin, and their combinations. PAR localization is measured as the sum intensity in the cytoplasm and normalized by the number of nuclei per field. (B) Intensity of nuclear PAR in SUM149PT cells treated for 4 h with OTI-611, olaparib, AZD5305, or doxorubicin, and their combinations. PAR mean intensity is normalized by the number of nuclei per field. Data expressed as the mean of three independent experiments ± S.E.M. (C) Representative images of PAR immunofluorescence showing changes in nuclear PAR and its translocation to the cytoplasm with OTI-611 treatment. Scale bar = 50 µm. (D) Intensity of cytoplasmic AIF in SUM149PT cells treated for 18 h with OTI-611, olaparib, AZD5305, or doxorubicin, and their combinations. (E) Intensity of nuclear AIF in SUM149PT cells treated for 18 h with OTI-611, olaparib, AZD5305, or doxorubicin, and their combinations. AIF mean intensity is normalized by the number of nuclei per field and expressed as the mean of two independent experiments ± S.E.M. (F) Representative images showing changes in cytoplasmic AIF and its translocation to the nucleus with OTI-611 treatment. Scale bar = 50 µm, * p < 0.05, ** p < 0.01, **** p < 0.0001.