Table 2.
Percentage relation between three forms of peptides in samples 1–5 (intact, oxidized and proteolytic).
| Peptides * | Escul 1 | Escul 1R | Brev 2L | Brev 2La | Brev 2Ra | Brev 2Rd | Brev 1Ra | Brev 1LE | Ranat 2R | |
|---|---|---|---|---|---|---|---|---|---|---|
| Forms | ||||||||||
| HCl (sample 1) | ||||||||||
| Intact, % | 38.61 | 64.86 | 98.79 | 30.98 | 98.98 | 99.61 | 93.51 | 100 | 85.21 | |
| Oxidized, % | 9.63 | 7.45 | - | - | - | - | 5.74 | - | - | |
| Proteoforms, % | 51.77 | 27.69 | 1.21 | 69.02 | 1.02 | 0.39 | 0.75 | - | 14.79 | |
| CH3OH (sample 2) | ||||||||||
| Intact, % | 79.99 | 77.85 | 95.34 | 100 | 100 | 100 | 98.27 | 100 | 100 | |
| Oxidized, % | - | - | - | - | - | - | 1.13 | - | - | |
| Proteoforms, % | 20.01 | 22.15 | 4.66 | - | - | - | 0.60 | - | - | |
| HCOOH (sample 3) | ||||||||||
| Intact, % | 72.22 | 84.41 | 95.26 | 95.02 | 98.70 | 97.77 | 99.51 | 98.37 | 65.01 | |
| Oxidized, % | 10.79 | - | - | - | - | - | 0.19 | - | - | |
| Proteoforms, % | 16.99 | 15.59 | 4.74 | 4.98 | 1.30 | 2.23 | 0.30 | 1.63 | 34.99 | |
| PMSF (0.1 mM) (sample 4) | ||||||||||
| Intact, % | 3.32 | 2.42 | 0.36 | - | 11.80 | 1.55 | 77.80 | 7.27 | - | |
| Oxidized, % | 2.28 | 0.25 | - | - | - | - | 22.20 | - | - | |
| Proteoforms, % | 94.40 | 97.33 | 99.64 | - | 88.21 | 98.45 | - | 92.73 | - | |
| PMSF (1.0 mM) (sample 5) | ||||||||||
| Intact, % | 8.10 | 0.74 | 19.49 | - | 85.99 | 20.84 | 99.47 | 10.26 | 100 | |
| Oxidized, % | - | - | - | - | - | - | - | - | - | |
| Proteoforms, % | 91.90 | 99.26 | 80.51 | - | 14.01 | 79.16 | 0.53 | 89.74 | - | |
* The data involve peptides listed in Table 1.