Table 1.
List of antibodies used for immunofluorescence and Western blotting.
| Antibody | Source | Dilution | Ref. | Supplier | RRID | Application |
|---|---|---|---|---|---|---|
| Calcineurin | Rabbit polyclonal | 1:300 | ab3673 | abcam, Cambridge, UK | AB_303991 | IF; WB |
| Human Tau | Chicken | 1:100 | ab75714 | abcam | AB_1310734 | IF |
| pTau (AT8) | Mouse | 1:100 | MN1020 | Thermo fisher | AB_223647 | IF; WB |
| GSK3beta | Mouse | 1:200 | ab93926 | abcam | AB_10563643 | IF |
| IgG | Goat anti-rabbit Alexa Fluor 594 | 1:400 | A-11012 | Invitrogen, Carlsbad, CA, USA | AB_2534079 | IF |
| IgG | Goat anti-mouse Alexa Fluor 488 | 1:400 | A-11001 | Invitrogen | AB_2534069 | IF |
| IgG | Goat anti-mouse Alexa Fluor 594 | 1.400 | A-11032 | Invitrogen | AB_2534091 | IF |
| IgY | Goat anti-chicken 488 | 1:400 | A-11039 | Invitrogen | AB_2534096 | IF |
| IgY | Goat anti-chicken 594 | 1:400 | A-11042 | Invitrogen | AB_2534099 | IF |
| T18 | Rabbit polyclonal | 1:1000 | in-house | WB | ||
| β-actin | Rabbit polyclonal | 1:5000 | ab8227 | abcam | AB_2305186 | WB |
| P62 | Rabbit monoclonal | 1:1000 | 8025 | Cell signaling, Danvers, MA, USA | AB_10859911 | WB |
| LC3A/B | Rabbit monoclonal | 1:1000 | ab228525 | abcam | AB_2827794 | WB |
| Atg3 | Rabbit polyclonal | 1:1000 | 3415 | Cell signaling | AB_2059244 | WB |
| Atg5 | Rabbit monoclonal | 1:1000 | Ab228525 | abcam | AB_2650499 | WB |
| Atg9 | Rabbit monoclonal | 1:1000 | Ab228525 | abcam | AB_10863880 | WB |
| Atg16L1 | Rabbit polyclonal | 1:1000 | Ab228525 | abcam | WB | |
| Beclin 1 | Rabbit monoclonal | 1:1000 | Ab228525 | abcam | AB_2692326 | WB |
| 6E10 | Mouse monoclonal | 1:1000 | 803002 | Biolegend, San Diego, CA, USA | AB_2564654 | WB |