Figure 3.

Differentiation between positive and negative signal populations in dPCR reactions using the first 6-plex generic reporter set in combination with a target panel for detection of KRAS and NRAS mutations and their corresponding WT controls. One-dimensional plots of all 6 fluorescence channels are shown. Details of the reporters used in each detection channel, including sequences, fluorophores and quenchers, can be seen in Table S1. Reaction chamber details from left to right: (1) NTC; (2–7) 10,000 cpr of one kind of target sequence each. A more detailed version of this figure, with samples containing all six targets at once, is shown in Figure S2. Separability scores between positive and negative droplet populations are indicated at the bottom of the legend.