Figure 5.

Quantitative PCR results of the first 6-plex generic reporter set in combination with a target panel for detection of KRAS and NRAS mutations and their corresponding WT controls. A background of 10,000 cpr of both WT targets is present in all reaction chambers aside from the NTC (reaction chamber 1). Single mutation targets were added in three different concentrations (1000 cpr, 100 cpr and 10 cpr). Above: One-dimensional plots of fluorescence channels detecting either NRAS WT in “Blue” or NRAS Q61K in “Red”. One-dimensional plots of all other fluorescence channels detecting the other targets are depicted in Figure S7. Below: Quantitative data of three replicates with reactions freshly prepared for each experiment. Expected concentrations, mean values and standard deviations of measured concentrations in cp/µL as well as mean values and standard deviations of the number of positive droplets in all three experiments are shown for each reaction chamber in each detection channel. Cells are color-coded wherever a positive result was expected for the specific chamber and channel. Separability scores between positive and negative droplet populations in the depicted reactions are indicated at the bottom.