Table 2.
Temperature [°C] | Duration [min:s] | Cycles | |
---|---|---|---|
Partitioning | 40 | 12:00 | - |
Initial denaturation | 95 | 3:00 | - |
Amplification | 95 | 0:15 | 60 |
58 | 0:60 | ||
Pressure release | 25 | 33:00 | - |
Temperature [°C] | Duration [min:s] | Cycles | |
---|---|---|---|
Partitioning | 40 | 12:00 | - |
Initial denaturation | 95 | 3:00 | - |
Amplification | 95 | 0:15 | 60 |
58 | 0:60 | ||
Pressure release | 25 | 33:00 | - |