Table 4.
Temperature [°C] | Duration [min:s] | Cycles | |
---|---|---|---|
Partitioning | 40 | 12:00 | - |
Initial denaturation | 95 | 5:00 | - |
Amplification | 95 | 0:10 | 20 |
60 | 0:30 | ||
Pressure release | 25 | 33:00 | - |
Temperature [°C] | Duration [min:s] | Cycles | |
---|---|---|---|
Partitioning | 40 | 12:00 | - |
Initial denaturation | 95 | 5:00 | - |
Amplification | 95 | 0:10 | 20 |
60 | 0:30 | ||
Pressure release | 25 | 33:00 | - |