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. 2024 Aug 6;22(8):357. doi: 10.3390/md22080357

Figure 5.

Figure 5

Nrf2 overexpression reverses the chemosensitizing activity of PXA. (A) Protein levels of Nrf2 in stably overexpressing empty vector (EV) and Nrf2 HCT116 and HT29 cells were examined by Western blotting. (B) Western blot assay to detect the effect of PXA in combination with CDDP on Nrf2 protein in EV and Nrf2 stable overexpressing HCT116 and HT29 cells. (C) CCK-8 assay was performed on EV and Nrf2 stable overexpressing HCT116 and HT29 cells treated with PXA and CDDP for 48 h. (D) The DNA damage levels of EV and Nrf2 stable overexpressing HCT116 and HT29 cells treated with PXA and CDDP for 24 h were evaluated using the comet assay. Scale bars: 100 μm. Results are expressed as means ± SD. * p < 0.05, ** p < 0.01 versus the EV group.