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Optimal and suboptimal specimens in terms of the RNA fragmentation index considering (a) RNA concentration and (b) storage time. Note that, under the concentration index of 10 ng/µL and >60 days of storage, samples were more likely to have a suboptimal RNA fragmentation index. The red line represents the percentage of optimal samples in terms of RNA fragmentation. Blue: optimal samples with RNA fragmentation index ≥ 0.14; orange: suboptimal samples with RNA fragmentation index < 0.14.
