Table 2.

Success rate (SR) of RNA-seq tested samples based on pre-analytical variables.

Site			SR
T			39/73 (53.4%)
N			4/11 (36.4%)
M	Bone	2/4 (50%)	11/18 (61.1%)
	Extraskeletal	9/14 (64%)
Sample type
Cell block			0/5 (0%)
Biopsy	US-guided	11/17 (64.7%)	35/58 (60.3%)
	EBUS/EUS	10/16 (62.5%)
	Thoracoscopic	5/9 (55.5%)
	CT-guided	6/12 (50%)
Surgical specimen	Lobectomy	5/17 (29.4%)	19/39 (48.7%)
	Wedge resection	10/15 (66.6%)
	Metastasectomy	4/7 (57.1%)
Fragmentation index
≥0.14			42/55 (76.4%)
<0.14			12/47 (25.5%)
Storage time (days)
≤30			22/33 (66.6%)
>30			32/69 (46.4%)