Reverse Phase HPLC chromatogram of Skeletonema marinoi pigments and derivatives in in vivo planktonic cells at 450 nm (a) (maximum absorbance wavelength of chlorophylls and carotenoids) and 405 nm (b) (maximum absorbance wavelength of pheophorbide a), and in ethanol extract at 450 nm (c) and 405 nm (d). The twelve major detected peaks, from the most polar to the most apolar, corresponded to (1) chlorophyll c2, (2) chlorophyll c1, (3) pheophorbide a, (3′) pheophorbide a isomer, (4) trans-fucoxanthin, (5) Neoxanthin, (6) cis-fucoxanthin, (7) diadinoxanthin, (8) diatoxanthin, (9) zeaxanthin, (10) chlorophyll a, (10’), pheophytin a, and (11) β,β-carotene. High peaks of pheophorbide a can be observed in the chromatograms of ethanol extracts.