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. 2024 Aug 6;12(8):1594. doi: 10.3390/microorganisms12081594

Figure 3.

Figure 3

Relative expression patterns of lincRNA-73240 in different chicken tissues/cells and subcellular localization of lincRNA-73240. (A) Relative expression levels of lincRNA-73240 in the cerebrum, cerebellum, proventriculus, muscles, harderian gland, bursa of Fabricius, ileum, thymus, cecum tonsils, spleen, liver, heart, and lungs. β-actin was the normalized gene and the cerebrum was as control group. Data expressed as mean ± SD of eight independent individuals; each experiment performed in triplicate; ANOVA test; different letters indicate significant differences (p < 0.05); same letters indicate insignificant differences (p > 0.05). (B) Relative expression levels of lincRNA-73240 in chicken HD11 macrophages, DF1 cells, and CEF cells. Data are shown as means ± SD; n = 4 independent experiments; ANOVA test; ns, not significant; *** p < 0.001; **** p < 0.0001. (C) Agarose gel electrophoresis was used to measure RT-qPCR amplicons of lincRNA-73240 in different tissues. Abbreviations: 1, cerebrum; 2, cerebellum; 3, proventriculus; 4, muscles; 5, harderian gland; 6, bursa of Fabricius; 7, ileum; 8, thymus; 9, cecum tonsils; 10, spleen; 11, liver; 12, heart; 13, lungs. (D) Agarose gel electrophoresis was used to measure RT-qPCR amplicons of lincRNA-73240 in different cells: 1, β-actin in CEF cells; 2, β-actin in DF1 cells; 3, β-actin in HD11 cells; 4, lincRNA-73240 in CEF cells; 5, lincRNA-73240 in CEF cells; 6, lincRNA-73240 in CEF cells. (E,F) Distribution of lincRNA-73240 in the nucleus and cytoplasm of HD11 macrophages (E) and APEC-infected HD11 macrophages (F). GAPDH and U6 are the marker genes of the cytoplasm and nucleus, respectively. Data are shown as means ± SD; n = 4 independent experiments; each experiment performed in triplicate; paired t test; *** p < 0.001; **** p < 0.0001.