Table 1.
Primers used for amplification of specific targeted regions.
| Target Region | Primer Name | Annealing Temperature (°C) | Sequence (5′–3′) | Expected Fragment Length (bp) | Reference |
|---|---|---|---|---|---|
| ompA | Rr190.70F | 51 | ATG GCG AAT ATT TCT CCA AAA | 632 | Mazhetese, et al. [58] |
| Rr190.701R | GTT CCG TTA ATG GCA GCA TCT | ||||
| ompB | ompBF | 50 | ACA TK*G TTA TAC ARA GTG Y*TA ATG C | 444 | Pillay and Mukaratirwa [47] |
| ompBR | CCG TCA TTT CCA ATA ACT AAC TC | ||||
| omp | 17kDaF | 50 | AAT GAG TTT TAT ACT TTA CAA AAT TCT AAA AAC CA | 450 | Pillay and Mukaratirwa [47] |
| 17kDaR | CAT TGT TCG TCA GGT TGG CG | ||||
| gltA | gltAF | 50 | ACC TAT ACT TAA AGC AAG TAT Y*GG T | 1234 | Pillay and Mukaratirwa [47] |
| gltAR | TCT AGG TCT GCT GAT TTT TTG TTC A |
* Degenerate bases ensure binding to all R. africae strains. Keto (K) can accommodate guanine (G) or thymine (T), while pyrimidine (Y) can accommodate cytosine (C) or thymine (T).