Figure 6.
Parasite burden in BALB/c mice infected with L. braziliensis and treated with 17-DMAG. BALB/c mice were infected in the ear with 105 promastigotes in the stationary phase of L. braziliensis and, after two weeks, treated intraperitoneally with 20 mg/kg/day of 17-DMAG or with 5% of glucose solution, as control, for two (A,B), four (C,D), or seven weeks (E,F). After each time point, the animals were euthanized and the ears (A,C,E) and retroauricular lymph nodes (B,D,F) were removed, macerated and the infected cells were plated into a 96-well plate to evaluate the parasitic burden in these organs using a limiting dilution assay. Mann–Whitney test, ** p < 0.01, *** p < 0.001.