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Visual limit of detection determination. (a) Visual LOD for bta-miR-103 using H-H (upper row) and T-T (lower row) assay formats. (b) Visual LOD for bta-miR-155 using H-H (upper row) and T-T (lower row) assay formats. (c) Visual LOD for bta-miR-215 using H-H (upper row) and T-T (lower row) assay formats. TLC spots were carried out in triplicate (n = 3).
