Figure 6. Inhibition of SeIN128 prolongs rolling and delays initiation of crawling.

(A) Time series of rolling probabilities of larvae with Basin activation (black), or SS04185 inhibition and Basin activation (red). Shaded regions show 95% confidential intervals of rolling probabilities. Genotypes: 13xLexAop2-IVS-CsChrimson::mVenus; R72F11-LexA/+; UAS- TeTxLC.tnt/+ (black); 13xLexAop2-IVS-CsChrimson::mVenus; R72F11-LexA/R54B01-Gal4.AD; UAS-TeTxLC.tnt/R46E07-Gal4.DBD (red). Genotypes in (B–F) are the same as mentioned here. (B) Rolling probabilities during first 5 s of stimulation in (A). Error bars, 95% confidence interval. n = 241, 164. Statistics: Chi-square test, χ² = 44.02, p < 0.001. (C) A violin plot of total time spent rolling for each individual larva during stimulation. Statistics: Mann–Whitney U test, p < 0.001, n = 221, 258. (D) Cumulative plot of rolling duration. Statistics: Mann–Whitney U test, p < 0.001, n = 160, 154. (E) A violin plot of end of first rolling bout for each larva during stimulation. Statistics: Mann–Whitney U test, p < 0.001, n = 160, 154. (F) A violin plot of start of first crawling bout for each larva during stimulation. Statistics: Mann–Whitney U test, p < 0.001, n = 65, 105. ***p < 0.001.

Figure 6—figure supplement 1. SeIN128 inhibition enhances rolling.

(A) Time series of rolling probabilities of larvae with Basin activation (black), or SS04185 inhibition and Basin activation (red). Larvae were incubated with heat to trigger the effect of shibire^ts1. The red bar and dashed lines display the window of optogenetic stimulation eliciting larval escape responses. Shaded areas show 95% confidential intervals of rolling probabilities. Genotypes: 13xLexAop2-IVS-CsChrimson::mVenus;R72F11-LexA/+; 20xUAS-TTS-Shibire/+ (black); 13xLexAop2-IVS-CsChrimson::mVenus; R72F11-LexA/R54B01-Gal4.AD; 20xUAS-TTS-Shibire/R46E07-Gal4.DBD (red). Genotypes in (B–G) are the same as mentioned here. (B) Binned larval rolling probabilities during first 5 s of stimulation in (A). Error bars, 95% confidence interval. n = 134, 143. Statistics: Chi-square test, χ² = 12.33, p < 0.001. (C) A violin plot of total time spent rolling for each individual larva during stimulation. Statistics: Mann–Whitney U test, p > 0.05, n = 85, 115. (D) Cumulative plot of rolling duration. Statistics: Mann–Whitney U test, p > 0.05, n = 219, 352. (E) A violin plot of start of first rolling bout for each larva during stimulation. Statistics: Mann–Whitney U test, p > 0.05, n = 85, 115. (F) A violin plot of end of first rolling bout for each larva during stimulation. Statistics: Mann–Whitney U test, p = 0.013, n = 85, 115. (G) A violin plot of start of first crawling bout for each larva during stimulation. Statistics: Mann–Whitney U test, p = 0.034, n = 32, 22. ***p < 0.001.