Figure 1.

Effect of 48 h vorinostat treatment, alone or in combination with N-acetyl-L-cysteine (NAC), on oxidative stress in (A) MKN-45 and (B,C) MKN-74 cells. The formation of reactive oxidative species (ROS) was determined by the increase in fluorescence of the ROS-sensitive reagent CellRox Deep Red. A right shift of the fluorescence distribution indicates increased ROS formation. Representative FACS analyses of three independent experiments are shown, with each experiment being performed in duplicates.