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. 2024 Aug 16;17(8):1080. doi: 10.3390/ph17081080

Figure 4.

Figure 4

(A) RNAi-mediated knockdown of Nrf2 in MKN-45 and MKN-74 cells, as determined on mRNA level. Cells were transfected with Nrf2-specific siRNA (siNrf2) or control siRNA (siLuc3), and Nrf2 expression was evaluated 48 h after transfection. (B,C) Effects of RNAi-mediated Nrf2 knockdown (siNrf2) on vorinostat toxicity, compared to a transfection with an unspecific control siRNA (siLuc3) or untreated cells (UT) in colony-formation assays. Scale bars indicate 5 mm. Viable colonies of (B) MKN-74 cells and (C) MKN-45 cells were stained with methylene blue. Representative images of three independent experiments are shown. (D) Effects of long-term vorinostat treatment (1 µM) and its modulation by Nrf2 knockdown (siNrf2) versus control siRNA (siLuc3), as determined in a formazan-based CCK8 assay for viable cells. Cells were treated for 5 days as indicated. Shown are mean values of three independent experiments + SEM. #, p > 0.05; *, p < 0.05 and **, p < 0.01.