Table 1.

Comparative quality metrics output by QUAST for the assemblies generated as part of this study and the reference assemblies available for each species

	Length (bp)	No. contigs	N50 (bp)	GC content	N content
Brugia malayi
Ghedin et al. (2007) reference assemblya	88,235,797	197	14,214,749	28.5%	0.30%
MinION data only assembly	88,513,498	88	4,666,641	28.43%	0%
MinION assembly polished with Illumina data	88,428,546	88	4,667,161	28.45%	0%
Hybrid assembly	84,528,932	49	3,943,761	28.57%	<0.001%
Trichuris trichiura
Foth et al. (2014) reference assemblyb	75,474,068	4,086	70,602	42.3%	0.30%
Doyle et al. (2022) reference assembly	80,573,711	113	11,299,416	42.3%	0.31%
MinION data only assembly	73,094,541	174	806,679	42.22%	0%
MinION assembly polished with Illumina data	72,959,001	174	805,117	42.23%	0%
Hybrid assembly	72,705,678	63	2,773,302	41.15%	0%
Ancylostoma caninum
International Helminth Genomes Consortium (2019) reference assemblyc	465,750,606	25,339	256,700	42.8%	13.50%
MinION data only assembly	358,159,610	1,578	638,901	43.36%	0%
MinION assembly polished with Illumina data	357,986,713	1,578	637,973	43.38%	0%
Hybrid assembly	348,034,555	652	1,095,227	43.24%	<0.001%

Abbreviations: bp = base pairs.

^aReference assembly included PacBio, optical mapping, and Sanger sequencing data.

^bReference assembly generated using Illumina data.

^cReference assembly generated using 454 sequencing.