Table 1.

DNA oligonucleotide primers and probes

Description	Sequence (5′-3′)	Name
rrp41-L187P SDM Fwd	GAAGAAAATGCTATGAGTACAGTGACACCAGGTGTGGTAGGGAAGTCAGAAAAAC	AC9500
rrp41-L187P SDM Rev	GTTTTTCTGACTTCCCTACCACACCTGGTGTCACTGTACTCATAGCATTTTCTTC	AC9501
Exosc4-L187P SDM Fwd	GCTGGAGGGCCCCAGCTCGCCCCGGCCCTGCTGCCCGCCTCCGGCC	AC9505
Exosc4-L187P SDM Rev	GGCCGGAGGCGGGCAGCAGGGCCGGGGCGAGCTGGGGCCCTCCAGC	AC9506
RRP41_565-gRNA Fwd	ATATGCATGCGAGTACAGTGACACTAGGTGGTTTTAGAGCTAGAAATAGC	AC9891
SUP4t-crRNA Rev	ATATGGTACCAGACATAAAAAACAAAAAAAGCACCACC	AC6809
rrp41-L187P-HDR Fwd	TACCAATTCATTAGAAGAAAATGCTATGAGTACAGTGACACCAGGTGTAGTAGGTAAGTCAGAAAAACTTTCTCTTTTATTGGTGGAA	AC9893
rrp41-L187P-HDR Rev	TTCCACCAATAAAAGAGAAAGTTTTTCTGACTTACCTACTACACCTGGTGTCACTGTACTCATAGCATTTTCTTCTAATGAATTGGTA	AC9894
EXOSC4 Exome Seq Fwd	GGAAGGGTGTGATGGGGTT	AM1
EXOSC4 Exome Seq Rev	GTGGGCAGAGGAGGGTTTTA	AM2
U4 pre-snRNA Fwd	AAAGAATGAATATCGGTAATG	AC5722
U4 pre-snRNA Rev	ATCCTTATGCACGGGAAATACG	AC5723
TLC1 pre-RNA Fwd	GTATTGTAGAAATCGCGCGTAC	AC7593
TLC1 pre-RNA Rev	CCGCCTATCCTCGTCATGAAC	AC7594
U14 snoRNA (snR128) Fwd	GATCACGGTGATGAAAGACTGG	AC5397
U14 snoRNA (snR128) Rev	CTACAGTATACGATCACTCAGACATCCTA	AC5398
ALG9 mRNA Fwd	CACGGATAGTGGCTTTGGTGAACAATTAC	AC5067
ALG9 mRNA Rev	TATGATTATCTGGCAGCAGGAAAGAACTTGGG	AC5068
P1 Probe (between A2 & A3 sites)	TGTTACCTCTGGGCCC	HG245
P2 Probe (between C2 & E sites)	GGCCAGCAATTTCAAGTTA	HG242
P3 Probe (5.8S rRNA)	CTGCGTTCTTGATCGATGCG	HG247
P4 Probe (between D & A2 sites)	GCTCTCATGCTCTTG CC	HG240
P5 Probe (18S rRNA)	CATGGCTTAATCTTTGAGAC	HG243
P6 Probe (25S rRNA)	GCCCGTTCCCTTGGCTGTG	HG244
scR1 Probe	ATCCCGGCCGCCTCCATCAC	HG315
5S Probe	CTACTCGGTCAGGCTC	HG246

The bolded nucleotides indicate codon mutations or PAM site mutations. The bolded sequence in RRP41_565-gRNA Fwd oligonucleotide is the gRNA sequence. The underlined sequences are restriction sites.