Table 2.
Saccharomyces cerevisiae strains and plasmids
| Strain/Plasmid | Description | Reference |
|---|---|---|
| BY4741 (ACY402) | MATa ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 | (60) |
| rrp41Δ (yAV2493) | MAT? ura3Δ0 leu2Δ0 his3Δ1 rrp41Δ::NEO [RRP41, URA3] | This study |
| rrp41-L187P (ACY3125) | MATa ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 rrp41-L187P | This study |
| rrp41-L187P (ACY3126) | MATa ura3Δ0 leu2Δ0 his3Δ1 met15Δ0 rrp41-L187P | This study |
| pRS315 | CEN6, LEU2, ampR | (51) |
| pRS425 | 2μ, LEU2, ampR | (52) |
| pAC2971 | RRP41 in pRS425, LEU2, 2μ | This study |
| pAC4179 | RRP41-Native 3′UTR in pRS315, CEN6, LEU2, ampR | This study |
| pAC4180 | rrp41-L187P-Native 3′UTR in pRS315, CEN6, LEU2, ampR | This study |
| pAC4242 | RRP41-2xMyc-Native 3′UTR in pRS315, CEN6, LEU2, ampR | This study |
| pAC4243 | rrp41-L187P-2xMyc-Native 3′UTR in pRS315, CEN6, LEU2, ampR | This study |
| pAC4397 | rrp41-L187P-Native 3′UTR in pRS425, 2μ, LEU2, ampR | This study |
| pAC4398 | RRP41-2xMyc-Native 3′UTR in pRS425, 2μ, LEU2, ampR | This study |
| pAC4399 | rrp41-L187P-2xMyc-Native 3′UTR in pRS425, 2μ, LEU2, ampR | This study |
| pcDNA3 | pCMV, NeoR, ampR | Invitrogen |
| pAC3517 | Myc-Exosc4 in pcDNA3, NeoR, ampR | This study |
| pAC4231 | Myc-Exosc4-L187P in pcDNA3, NeoR, ampR | This study |
| pAC3846 | TEF1p-Cas9-CYC1t-SNR52p in pRS316, CEN6, URA3, ampR | (48) |
| pAC4339 | TEF1p-Cas9-CYC1t-SNR52p-RRP41_565.gRNA-SUP4t in pRS316, CEN6, URA3, ampR | This study |